       Document 0793
 DOCN  M9460793
 TI    Detection of multiple 'Ebnotypes' in individual Epstein-Barr virus
       carriers following lymphocyte transformation by virus derived from
       peripheral blood and oropharynx.
 DT    9404
 AU    Gratama JW; Oosterveer MA; Weimar W; Sintnicolaas K; Sizoo W; Bolhuis
       RL; Ernberg I; Department of Clinical and Tumour Immunology, Daniel den
       Hoed; Cancer Centre, Rotterdam, The Netherlands.
 SO    J Gen Virol. 1994 Jan;75 ( Pt 1):85-94. Unique Identifier : AIDSLINE
       MED/94157478
 AB    Transformation of a B lymphocyte into a lymphoblastoid cell line (LCL)
       by Epstein-Barr virus (EBV) results in the expression of EBV nuclear
       antigens (EBNAs) of which the size spectrum ('Ebnotype') is
       characteristic for the transforming virion. Ebnotyping has been used as
       an epidemiological tool for studies of EBV infection. We compared the
       occurrence of a single and of multiple Ebnotypes, as defined by EBNAs 1,
       2 and 6, in healthy and diseased EBV carriers. Cases from which two or
       more LCLs could be established from peripheral blood or oropharyngeal
       cultures were considered informative. The frequency of multiple
       Ebnotypes was relatively low in healthy individuals and in patients with
       infectious mononucleosis or with haematological diseases who were
       awaiting a bone marrow transplant [blood, 11 of 74 patients (15%);
       oropharynx, 12 of 49 patients (24%)], whereas it was relatively high in
       recipients of bone marrow or cardiac allografts and one patient with
       AIDS [blood, 12 of 34 patients (35%); oropharynx, 11 of 16 patients
       (69%)]. Three patterns of the simultaneous presence of multiple
       Ebnotypes were distinguished. The first, most frequent, pattern observed
       predominantly in oropharyngeal cultures of all groups consisted of
       minority Ebnotypes differing from the majority type by only a single
       EBNA protein (usually EBNA 1). The second, less frequent, pattern
       observed in the healthy carriers and the (candidate) transplant
       recipients consisted of minority Ebnotypes differing from the majority
       type by two EBNA proteins (mostly EBNAs 1 and 6). The third pattern,
       characterized by the simultaneous presence of totally different
       Ebnotypes, was restricted to the (candidate) transplant recipients and
       the AIDS patient and was more frequently observed in the blood than in
       the oropharynx. We suggest that the first two patterns result from
       heterologous recombinations occurring during viral replication at repeat
       sequences within the EBNA coding regions, whereas the third pattern
       reflects multiple infections with exogenous viruses.
 DE    Acquired Immunodeficiency Syndrome/MICROBIOLOGY  Antigens,
       Viral/ANALYSIS/*GENETICS  Blood/*MICROBIOLOGY  Carrier
       State/*MICROBIOLOGY  Cell Line  DNA-Binding Proteins/ANALYSIS/*GENETICS
       Herpesviridae Infections/*MICROBIOLOGY  Herpesvirus 4,
       Human/*GENETICS/ISOLATION & PURIF  Human  Infectious
       Mononucleosis/MICROBIOLOGY  Lymphocyte Transformation
       Oropharynx/*MICROBIOLOGY  Support, Non-U.S. Gov't  Transplantation
       Tumor Virus Infections/MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

