       Document 0544
 DOCN  M9460544
 TI    A macrophage differentiating factor derived from human T cell line
       HUT102 acting on a mouse myeloid cell line M1.
 DT    9404
 AU    Kanno H; Nose M; Niki T; Miyazawa M; Kyogoku M; Department of Pathology,
       Tohoku University School of Medicine,; Sendai.
 SO    Tohoku J Exp Med. 1993 Sep;171(1):43-52. Unique Identifier : AIDSLINE
       MED/94167747
 AB    Human T cell leukemia virus type I-transformed T cell line HUT102
       constitutively secreted soluble factors which induced differentiation of
       a murine myeloid leukemic cell line, M1, to increase the immune
       complex-binding and/or phagocytizing capacity. This macrophage
       differentiating factor(s) (MDF) was purified from the culture
       supernatants of HUT102 cells by using several steps of column
       chromatography and novel immune-adherence and/or immune-phagocytic
       assays. The finally purified MDF activity was detected in the fraction
       that consisted of 40,000- and 45,000- molecular weight molecules.
       Antibodies specific for human interleukin-6 or for human
       granulocyte-colony stimulating factor, both of which have
       differentiation-inducing activity on M1 cells when used as a single
       factor, could not neutralize the MDF activity. These findings suggest
       that the 40,000- and/or 45,000- molecular weight molecules in the HUT102
       cell products may be possible novel differentiation-inducing factors
       acting on a murine macrophage lineage across the species barrier.
 DE    Animal  Cell Differentiation/*DRUG EFFECTS  Cell Line, Transformed
       Chromatography, Affinity  Chromatography, High Pressure Liquid
       Chromatography, Ion Exchange  Cycloheximide/PHARMACOLOGY
       Cytokines/IMMUNOLOGY  Human  HTLV-I  Macrophages/*PHYSIOLOGY  Mice
       Mice, Inbred Strains  Molecular Weight  Support, Non-U.S. Gov't  Tumor
       Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

