       Document 0399
 DOCN  M9460399
 TI    Phenotypic analysis of lymphocytes obtained by bronchoalveolar lavage of
       normal sheep.
 DT    9404
 AU    Burrells C; Sutherland AD; Moredun Research Institute, Edinburgh, UK.
 SO    Vet Immunol Immunopathol. 1994 Jan;40(1):85-90. Unique Identifier :
       AIDSLINE MED/94174704
 AB    Lungs were excised from apparently healthy sheep at slaughter and
       lymphocytes obtained by bronchoalveolar lavage were separated by buoyant
       density and characterised phenotypically. In most animals T-lymphocytes
       (CD5+ cells) accounted for almost the entire population, with
       B-lymphocytes (sIg+ cells) generally accounting for less than 10%. In
       the main, CD4+ T cells exceeded CD8+ T cells by at least a factor of
       three and CD4-/CD8- (gamma delta) T cells represented around 6% of the
       population. Proportions of subtypes of lymphocytes obtained by
       bronchoalveolar lavage differed from those in peripheral blood. The
       normal range of lymphocyte phenotypes in the ovine lung established in
       this study will be of value in subsequent work on animals with defined
       respiratory disease.
 DE    Animal  Antibodies, Monoclonal  Antigens, CD/IMMUNOLOGY
       B-Lymphocytes/*IMMUNOLOGY  Bronchoalveolar Lavage
       Fluid/CYTOLOGY/*IMMUNOLOGY  CD4-CD8 Ratio  Flow Cytometry/VETERINARY
       Immunophenotyping/*VETERINARY  Leukocyte Count  Sheep/*IMMUNOLOGY
       T-Lymphocytes/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

