       Document 0398
 DOCN  M9460398
 TI    The kinetics of human immunodeficiency virus reverse transcription are
       slower in primary human macrophages than in a lymphoid cell line.
 DT    9404
 AU    Collin M; Gordon S; Sir William Dunn School of Pathology, University of
       Oxford,; United Kingdom.
 SO    Virology. 1994 Apr;200(1):114-20. Unique Identifier : AIDSLINE
       MED/94174708
 AB    Reverse transcription is a critical event in the life of a retrovirus
       and a potential determinant of viral infectivity. The kinetics of
       endogenous reactions are relatively well defined but little is known
       about HIV reverse transcription during infection. In this report, we
       have estimated the rate and efficiency of HIV-1 reverse transcription in
       primary macrophages and H9 lymphoid cells using quantitative PCR to
       detect intermediate cDNA structures. DNA synthesis is completed 12-16 hr
       after infection of H9 cells, but requires more than 36 hr in M phi,
       owing to slower rates of extension and template switching. Reverse
       transcription was inefficient in both cell types and in H9 cells the
       kinetics were sufficiently well resolved to estimate that only one in
       three transcripts initiated were extended to full-length viral DNA.
       Slower DNA synthesis largely accounts for the longer replicative cycle
       of HIV in macrophages. The rate of reverse transcription, which may
       depend upon levels of deoxynucleotide triphosphate substrates, is a
       potential factor in modulating the permissiveness of macrophage
       populations in vivo.
 DE    Base Sequence  Cells, Cultured  Comparative Study  DNA,
       Complementary/BIOSYNTHESIS  DNA, Viral/BIOSYNTHESIS  Human
       HIV-1/*METABOLISM  Kinetics  Lymphocytes/*MICROBIOLOGY
       Macrophages/*MICROBIOLOGY  Molecular Sequence Data  Polymerase Chain
       Reaction  Reverse Transcriptase/*METABOLISM  Support, Non-U.S. Gov't
       *Transcription, Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

