       Document 0292
 DOCN  M9460292
 TI    Stability of infectious HIV in clinical samples and isolation from small
       volumes of whole blood.
 DT    9404
 AU    O'Shea S; Rostron T; Mullen JE; Banatvala JE; Department of Virology
       United Medical Schools, Guys Hospital,; London.
 SO    J Clin Pathol. 1994 Feb;47(2):152-4. Unique Identifier : AIDSLINE
       MED/94179557
 AB    AIMS--To evaluate the stability of infectious HIV in clinical samples
       and the efficiency of isolating it from small volumes of whole blood.
       METHODS--Titres of infectious HIV were measured in peripheral blood
       mononuclear cells and plasma 2, 24, and 48 hours after peripheral blood
       had been collected from 10 HIV positive adult volunteers. Volumes of
       whole blood (1 ml to 5 microliters), collected from a further five
       volunteers, were used to determine the minimum volume from which HIV
       could be isolated. Infectious HIV was isolated by co-culture with
       phytohaemagglutinin stimulated umbilical cord mononuclear cells.
       RESULTS--Geometric mean titres of infectious HIV seemed to be more
       stable in peripheral blood mononuclear cells than in plasma. HIV was
       recovered from all 10 peripheral blood mononuclear cell samples during
       the 48 hours after sample collection, but from only four plasma samples.
       HIV could occasionally be isolated from 5 microliters of whole blood and
       reliably from 200 microliters. CONCLUSIONS--HIV can be isolated from
       peripheral blood mononuclear cells and plasma for up to 48 hours after
       sample collection. Isolation of HIV from small volumes of whole blood
       has applications for the diagnosis and management, of HIV positive
       children.
 DE    Adult  Blood/*MICROBIOLOGY  *Blood Preservation  Blood Specimen
       Collection  Human  HIV/*ISOLATION & PURIF  Leukocytes,
       Mononuclear/*MICROBIOLOGY  Plasma/MICROBIOLOGY  Support, Non-U.S. Gov't
       Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

