       Document 0265
 DOCN  M9460265
 TI    A simple and reliable method to detect cell membrane proteins on
       infectious human immunodeficiency virus type 1 particles.
 DT    9404
 AU    Capobianchi MR; Fais S; Castilletti C; Gentile M; Ameglio F; Dianzani F;
       Institute of Virology, University La Sapienza, Rome, Italy.
 SO    J Infect Dis. 1994 Apr;169(4):886-9. Unique Identifier : AIDSLINE
       MED/94179905
 AB    Identification of cell-derived molecules on infectious human
       immunodeficiency virus type 1 (HIV-1) particles may be helpful in
       investigating mechanisms of HIV infection and in vaccine studies. Some
       of these molecules were detected on HIV-1 virions in previous studies,
       but rather elaborate methods were used. The method presented here allows
       an extensive characterization of the cell surface molecules associated
       with HIV-1 by capturing virus particles on monoclonal antibodies to cell
       membrane antigens bound to plastic wells. Binding of infectious virus
       was assessed by adding permissive target cells (C8166) and determining
       viral replication. With this procedure, beta 2-microglobulin, HLA-DR,
       intercellular adhesion molecule-1, and leukocyte function antigen-1 were
       found on HIV-1 particles from laboratory strains and primary clinical
       isolates. In contrast, CD19, CD4, and CD8 molecules were not detected.
 DE    beta 2-Microglobulin/ANALYSIS  Antigens, Surface/ANALYSIS  Antigens,
       Viral/*ANALYSIS  Cell Adhesion Molecules/ANALYSIS  Cell Line  Cell
       Membrane/CHEMISTRY/IMMUNOLOGY  Enzyme-Linked Immunosorbent Assay  Human
       HIV-1/*CHEMISTRY/IMMUNOLOGY/ULTRASTRUCTURE  HLA-DR Antigens/ANALYSIS
       Lymphocyte Function-Associated Antigen-1/ANALYSIS  Support, Non-U.S.
       Gov't  Viral Matrix Proteins/*ANALYSIS
       Virion/*CHEMISTRY/IMMUNOLOGY/ULTRASTRUCTURE  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

