       Document 0209
 DOCN  M9460209
 TI    A transforming fragment within the direct repeat region of human
       herpesvirus type 6 that transactivates HIV-1.
 DT    9404
 AU    Thompson J; Choudhury S; Kashanchi F; Doniger J; Berneman Z; Frenkel N;
       Rosenthal LJ; Department of Microbiology, Georgetown University School
       of; Medicine, Washington DC 20007.
 SO    Oncogene. 1994 Apr;9(4):1167-75. Unique Identifier : AIDSLINE
       GENBANK/X73675
 AB    HHV-6 infection has been associated with several malignancies including
       non-Hodgkin's lymphoma and Hodgkin's disease by the presence of high
       antibody titer and/or the presence of HHV-6 DNA. To understand their
       oncogenic potential, SalI restriction fragments from HHV-6 strain U1102
       were transfected into NIH3T3 cells to assess transforming ability. A
       3.9-kbp SalI-L DNA fragment spanning the junction of the direct repeat
       left (DRL) and unique long segment (UL) regions of HHV-6 induced foci of
       morphologically altered cells. The SalI-L transformed NIH3T3 focal lines
       induced tumors in nude mice within 2 weeks. The retention of HHV-6
       specific DNA observed in SalI-L transformed cells and their
       tumor-derived lines suggest a possible maintenance function. Since both
       HHV-6 infection as well as transforming fragments from other DNA viruses
       have been shown to transactivate the human immunodeficiency virus type 1
       (HIV-1) long terminal repeat (LTR), SalI-L was examined for
       transactivation activity. SalI-L up-regulated HIV-1 LTR CAT 10-15 fold
       in both monkey CV-1 and human T Jurkat cells. The further study of the
       SalI-L transforming fragment exhibiting transactivation of HIV-1 LTR
       will elucidate whether these two activities are encoded by a single gene
       and will aid in the understanding of the interaction between HHV-6 and
       HIV-1 as it relates to progression of AIDS and/or AIDS-related
       malignancies.
 DE    Animal  Base Sequence  *Cell Transformation, Neoplastic  Haplorhini
       Herpesvirus 6, Human/*GENETICS  Human  *HIV Long Terminal Repeat  Mice
       Mice, Nude  Molecular Sequence Data  Neoplasms, Experimental/GENETICS
       Restriction Mapping  Sequence Homology, Nucleic Acid  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  *Trans-Activation (Genetics)
       Transfection  Tumor Cells, Cultured  3T3 Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

