       Document 0113
 DOCN  M9460113
 TI    Functional role of the V1/V2 region of human immunodeficiency virus type
       1 envelope glycoprotein gp120 in infection of primary macrophages and
       soluble CD4 neutralization.
 DT    9404
 AU    Koito A; Harrowe G; Levy JA; Cheng-Mayer C; Department of Medicine,
       University of California, San Francisco; 94143-0128.
 SO    J Virol. 1994 Apr;68(4):2253-9. Unique Identifier : AIDSLINE
       MED/94187065
 AB    We have examined the influence of the V1/V2 region of the human
       immunodeficiency virus type 1 (HIV-1) gp120 on certain biologic
       properties of the virus. We observed that on the genomic background of
       the T-cell-line-tropic strain, HIV-1SF2mc, both the V1 and V2 domains of
       the macrophage-tropic strain, HIV-1SF162mc, in addition to the required
       V3 domain, are necessary to attain full macrophage tropism. Furthermore,
       the V2 domain modulates the sensitivity of HIV-1 to soluble CD4
       neutralization. Structural studies of recombinant and mutant envelope
       glycoproteins suggest that the function of the V1/V2 region is to
       interact with the V3 domain and confer on the envelope gp120 of
       HIV-1SF2mc a conformation more similar to that of the macrophage-tropic
       strain HIV-1SF162mc. The conformation of the envelope gp120 appears to
       be strain specific and plays an important role in determining HIV-1
       tissue tropism and sensitivity to soluble CD4 neutralization.
 DE    Amino Acid Sequence  Animal  Antigens, CD4/*IMMUNOLOGY  Comparative
       Study  Gene Products, env/BIOSYNTHESIS/GENETICS  Glycosylation
       Haplorhini  Human  HIV Envelope Protein gp120/*GENETICS/METABOLISM
       HIV-1/*GROWTH & DEVELOPMENT/*IMMUNOLOGY/PATHOGENICITY
       Macrophages/*MICROBIOLOGY  Molecular Sequence Data  Mutation
       Neutralization Tests  Protein Precursors/BIOSYNTHESIS/GENETICS  Protein
       Processing, Post-Translational/GENETICS  Recombination, Genetic
       Sequence Homology, Amino Acid  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  Virulence/GENETICS  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

