       Document 0109
 DOCN  M9460109
 TI    Retrovirus recombination depends on the length of sequence identity and
       is not error prone.
 DT    9404
 AU    Zhang J; Temin HM; McArdle Laboratory for Cancer Research, University
       of; Wisconsin-Madison 53706.
 SO    J Virol. 1994 Apr;68(4):2409-14. Unique Identifier : AIDSLINE
       MED/94187082
 AB    Retroviruses, as a result of the presence of two identical genomic RNA
       molecules in their virions, recombine at a high rate. When nonhomologous
       RNA is present in the dimer RNA molecules, nonhomologous recombination
       can occur, although the rate is very low, only 0.1% of the rate of
       essentially homologous recombination (J. Zhang and H. M. Temin, Science
       259:234-238, 1993). We found, as is found in naturally occurring highly
       oncogenic retroviruses (J. Zhang and H. M. Temin, J. Virol.
       67:1747-1751, 1993), that the crossovers usually occur at a short region
       of sequence identity. We modified the previously studied vectors to
       study the effect of different lengths of short regions of sequence
       identity in the midst of otherwise nonidentical sequences. We found that
       the efficiency of recombination depends on the length of this sequence
       identity. However, the highest rate in such molecules remained lower
       than for recombination between essentially homologous molecules, even
       when there was extensive sequence identity. Junction sequences of the
       recombinants indicated that retrovirus recombination is not an
       error-prone process as was reported for human immunodeficiency virus
       reverse transcriptase by using a cell-free system (J. A. Peliska and S.
       J. Benkovic, Science 258:1112-1118, 1992).
 DE    Base Sequence  Genetic Vectors/GENETICS  Molecular Sequence Data
       Moloney Leukemia Virus/GROWTH & DEVELOPMENT/*GENETICS  Mutagenesis
       Proviruses/GENETICS  *Recombination, Genetic  RNA/GENETICS  RNA,
       Antisense/GENETICS  RNA, Viral/BIOSYNTHESIS/*GENETICS  *Sequence
       Homology, Nucleic Acid  Support, U.S. Gov't, P.H.S.  Virion/GROWTH &
       DEVELOPMENT  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

