       Document 0093
 DOCN  M9460093
 TI    Murine retrovirus-induced depletion of T cells is mediated through
       activation-induced death by apoptosis.
 DT    9404
 AU    Saha K; Yuen PH; Wong PK; Science Park-Research Division, University of
       Texas, M.D.; Anderson Cancer Center, Smithville 78957.
 SO    J Virol. 1994 Apr;68(4):2735-40. Unique Identifier : AIDSLINE
       MED/94187116
 AB    ts1, a mutant of Moloney murine leukemia virus, causes neurologic
       disorders and acute immunodeficiency associated with the destruction of
       thymocytes and helper T cells. In this study, we examined whether
       apoptosis was involved in ts1-induced killings of T cells. Neonatal mice
       were inoculated with ts1, and 20 to 23 days postinoculation, when
       cytopathic effects on T cells normally appear, thymocytes and splenic
       lymphocytes were isolated and examined. Our results showed that several
       features of apoptosis were present in ts1-infected thymocytes and
       splenic lymphocytes. Apoptotic fragmented DNA, condensation of the
       chromatin, and enhanced cell death after stimulation with mitogens which
       was preventable with protein synthesis inhibitors, all of which are
       common features of apoptotic cell death, were observed in ts1-infected
       cells. Several other viruses, including human immunodeficiency virus,
       have been shown to cause apoptotic death of T cells. Here we show for
       the first time that a murine retrovirus which also induces
       immunodeficiency can cause apoptotic T-cell death. Future studies with
       this murine retrovirus may provide important results to help us better
       understand the mechanisms of retrovirus-induced apoptosis of T cells.
 DE    Animal  Animals, Newborn  *Apoptosis  DNA Damage  Lymphocyte
       Transformation  Mice  Mice, Inbred Strains  Moloney Leukemia
       Virus/*PATHOGENICITY  Retroviridae Infections/*PATHOLOGY
       Spleen/PATHOLOGY  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/*PATHOLOGY/ULTRASTRUCTURE  Thymus
       Gland/PATHOLOGY/ULTRASTRUCTURE  Tumor Virus Infections/*PATHOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

