       Document 0045
 DOCN  M9460045
 TI    Multifunctional immunological monitoring of HIV positive patients: a
       novel staging system.
 DT    9404
 AU    del Llano AM; Zorrilla C; Lavergne JA; Department of Biology, University
       of Puerto Rico.
 SO    P R Health Sci J. 1993 Dec;12(4):277-81. Unique Identifier : AIDSLINE
       MED/94188472
 AB    A tri-functional in vitro evaluation has been utilized to analyze
       peripheral blood mononuclear cells (BMNC) from HIV-infected patients,
       which allows for the classification of these individuals into convenient
       stages, according to the number of in vitro parameters affected. The
       classifying functional parameters are: the mitochondrial metabolic
       activity of freshly isolated BMNC, measured by an MTT reduction assay,
       the detection of apoptosis in 72 hour cultures of these cells assessed
       by propidium iodide staining and dual parametric flow cytometric
       analysis, and their proliferative response to pokeweed mitogen. Our
       results indicate that HIV-infected patients at different stages of their
       clinical disease, can present dysfunctions in one, two or three of the
       above-mentioned parameters. Based on these results, patients can be
       classified into four newly-described stages which are Stage 0, including
       uninfected controls and all patients with unaffected parameters, and
       Stages 1, 2 and 3, including patients having one, two or all three
       parameters affected, respectively. This type of immunological evaluation
       and classification of HIV-infected patients has the potential of
       becoming a predictive tool in the longitudinal follow-up of their HIV
       infection.
 DE    Apoptosis  Colorimetry  Female  Flow Cytometry  Human  HIV
       Seropositivity/*CLASSIFICATION/*PHYSIOPATHOLOGY  Immunologic Tests
       Leukocytes, Mononuclear/METABOLISM  Prognosis  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

