       Document 0033
 DOCN  M9460033
 TI    Enzyme immunoassays (EIAs) for the detection of anti-Haemophilus ducreyi
       serum IgA, IgG, and IgM antibodies.
 DT    9404
 AU    Roggen EL; Hoofd G; Van Dyck E; Piot P; Department of Infection and
       Immunity, Institute of Tropical; Medicine, Antwerp, Belgium.
 SO    Sex Transm Dis. 1994 Jan-Feb;21(1):36-42. Unique Identifier : AIDSLINE
       MED/94188783
 AB    BACKGROUND AND OBJECTIVES: Chancroid is a risk factor for heterosexually
       acquiring HIV. Controlling its spread may reduce HIV transmission. GOAL
       OF THE STUDY: To develop EIAs for assessing antibody levels and for
       seroepidemiologic studies. STUDY DESIGN: Anti-Haemophilus ducreyi IgA,
       IgG and IgM EIAs were standardized using a crude cocktail antigen.
       Evaluation was on sera from Kenya, Rwanda, Thailand and The Gambia. The
       two-tailed student's t test was used to compare results. RESULTS: The
       specificity of IgA was 97% (95% confidence interval (CI): 95-99%), of
       IgG was 92% (95% CI: 89-95%), and of IgM was 99% (95% CI: 98-100%). The
       sensitivity of IgA was 88% (95% CI: 83-93%), of IgG was 93% (95%
       CI:89-97%), and of IgM was 78% (95% CI:71-85%) in patients having an
       ulceration for more than eight days. Thus, 95% (95% CI:92-98%) of the
       chancroid patients were seropositive for at least one antibody type. The
       IgG and IgA EIAs were more sensitive in patients older than 24 years of
       age. Higher IgG rates were found in HIV infected chancroid patients.
       CONCLUSION: The EIAs should be useful for studying the kinetics of
       antibody levels and the epidemiology of H. ducreyi infection.
 DE    Adolescence  Adult  Antibodies, Anti-Idiotypic/*IMMUNOLOGY  Antibodies,
       Bacterial/*IMMUNOLOGY  Chancroid/COMPLICATIONS/DIAGNOSIS/IMMUNOLOGY
       Enzyme-Linked Immunosorbent Assay  Female  Haemophilus
       ducreyi/*IMMUNOLOGY  Human  HIV Infections/TRANSMISSION  IgA/*IMMUNOLOGY
       IgG/*IMMUNOLOGY  IgM/*IMMUNOLOGY  Immunoenzyme Techniques  Male  Middle
       Age  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

